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Hepatitis B virus (HBV) is an important public health problem. Nowadays, the most widely used methods for HBV detection are nucleic acid tests, which have some drawbacks, such as time consumption, high cost, multiple steps and complicated steps. A one-step real-time fluorescent assay with high sensitivity and specificity for HBV detection is in demand. In this study, a novel one-step real-time fluorescent assay based on gold nanoparticles-hyaluronic acid (AuNPs-HA) complex was developed for specific detection of HBV in a single homogeneous step. HA, due to its high stability and non-toxicity, was used as a stabilizer for AuNPs. The AuNPs-HA complex, which contained AuNPs and HA, was used as a fluorescent probe for specific detection of HBV DNA. The fluorescent intensity of the AuNPs-HA complex was highly dependent on the concentration of the target DNA and existed in a wide linear range from 0.01 to 10 µg/mL with a detection limit of 3.28 ng/mL. When applied to clinical samples, the HBV detection results were consistent with ELISA and PCR results. This methodology holds promise as a powerful tool for on-site detection of HBV DNA in a closed single-tube format.
What happened to Browsershots? — josh_fyi

======
theaccordance
This was my only use of Browsershots and I never heard about it again. It was
a neat idea, and I’d love to know more about it. Anyone out there knows how
much he or she spent to get it going? I’d love to follow in your footsteps
and get back to the community.

~~~
dzorzky
Someone must have. It was a month ago that they had just under 400 screenshots
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